Composite

Part:BBa_K1194001:Design

Designed by: Aman Kumar   Group: iGEM13_IIT_Madras   (2013-09-26)


pLuxR --> I3A gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 311
    Illegal AgeI site found at 674
    Illegal AgeI site found at 1767
    Illegal AgeI site found at 2281
    Illegal AgeI site found at 2551
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lee JH, Kim YG, Kim CJ, Lee JC, Cho MH, Lee, "Indole-3-acetaldehyde from Rhodococcus sp. BFI 332 inhibits E.coli O157:H7 biofilm formation" Appl Microbiol Biotechnol 2012 Nov:96(4):1071-8



Source

The gene sequence for tryptophan transaminase is derived from Arabidopsis Thaliana. The gene sequence for indole pyruvate decarboxylase is taken from Azospirillum brasilense. The sequence for pLuxR is taken from Registry.

References