Composite
Part:BBa_K1194001:Design
Designed by: Aman Kumar Group: iGEM13_IIT_Madras (2013-09-26)
pLuxR --> I3A gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 253
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 311
Illegal AgeI site found at 674
Illegal AgeI site found at 1767
Illegal AgeI site found at 2281
Illegal AgeI site found at 2551 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Lee JH, Kim YG, Kim CJ, Lee JC, Cho MH, Lee, "Indole-3-acetaldehyde from Rhodococcus sp. BFI 332 inhibits E.coli O157:H7 biofilm formation" Appl Microbiol Biotechnol 2012 Nov:96(4):1071-8
Source
The gene sequence for tryptophan transaminase is derived from Arabidopsis Thaliana. The gene sequence for indole pyruvate decarboxylase is taken from Azospirillum brasilense. The sequence for pLuxR is taken from Registry.